TR4

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nuclear receptor subfamily 2, group C, member 2
TR4

PDB ID : 3P0U
PDB3P0U
HGNC(alias)NR2C2 (TAK1,TR2R1,hTAK1,TR4)
Gene CardNR2C2
Entrez7182
RefSeqNM_003298
UniProtP49116
UCSCBrowser view
WikipediaNR2C2
Protein FamilyGlucocorticoid receptor-like (DNA-binding domain)
TypePol II TF
Ensembl Exp.Human

Function

TR4 (testicular receptor 4) is a member of the nuclear hormone receptor family 2 group C (NR2C2), and acts as ligand-activated transcription factor. It has an N-terminal transactivation domain, a central DNA-binding domain with 2 zinc fingers, and a ligand-binding domain at the C-terminus. TR4 binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences, and can act as a repressor or activator of transcription. TR4 and NR2C1 form the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription (including that of GATA1). TR4 plays a fundamental role in early embryonic development and embryonic stem cells, and is required for spermatogenesis and cerebellum development. It is an important repressor of nuclear receptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor, and estrogen receptor pathways. TR4 also activates transcription of LHCG, and is an antagonist of PPARA-mediated transactivation.

ENCODE ChIP-seq Datasets

Indicated in the matrix are the numbers of datasets specified by lab and cell line; when the number is greater than 1, multiple ChIP-seq experiments have been performed, some upon treatments. Click the numbers to download the data files on ChIP-seq peaks, alignments, etc.

+/-
USC
GM12878 1
HeLa-S3 1
HepG2 1
K562 1


Average Profiles of Modified Histones around the Summit of ChIP-seq Peaks

Average histone modification profiles are shown for the [-2 kb, +2 kb] window around the summits of TF ChIP-seq peaks, separately for peaks that are proximal ([-1kb, +1kb]) to an annotated transcript (dashed lines) start sites and for peaks that are distal (more than 1kb) to all annotated transcripts (solid lines) start sites. Proximal profiles are arranged such that the transcriptional direction of the nearest transcript is toward the right. Histone modification data were generated by the Broad team, using antibodies to pull down modified histones followed by deep sequencing of the genomic DNA associated with the modified histones. Only histone modification data from the same cell line as the TF ChIP-seq data are shown.

Mouse over a curve to reveal its identity. Mouse over a histone modification in the legend to show its curves and gray out other histone modifications in the figures. Click a histone modification in the legend to toggle on/off its curve in all figures. Click the “Proximal” or “Distal” button in the legend to show only the average histone modification profiles anchored around ChIP-seq peaks that are proximal or distal to annotated transcripts.

Average Profiles of Nucleosomes around the Summit of ChIP-seq Peaks

Average nucleosome occupancy profiles are shown for the [-2 kb, +2 kb] window around the summits of TF ChIP-seq peaks, separately for peaks that are proximal to an annotated transcript (red lines) and for peaks that are distal to all annotated transcripts (blue lines), as defined in the previous section. Nucleosome positioning data in GM12878 and K562 were generated by the Stanford team, with micrococcal nuclease digestion of chromatin followed by deep sequencing of mononucleosomal DNA.

Motifs Enriched in the Top 500 ChIP-seq Peaks

The sequences of the top 500 TF ChIP-seq peaks were used to identify enriched motifs de novo, using the MEME-ChIP suite of tools. Five motifs are reported (M1 to M5), with motif name, sequence logo, and number of peaks out of the top 500 peaks that contain a site for the motif. The motifs are then used to scan the entire set of ChIP-seq peaks and the two flanking (control) regions using the FIMO tool, and two quantities are reported for bins of peaks sorted by their ChIP-seq q-values: percentage of the peaks that contain a site for the motif, and the distribution of the distances of the motif site to the summit of the peak.

Select MotifCell Line:   Lab:   Protocol:   Treatment:   Antibody:  

Cell Line:GM12878   Lab:USC   Protocol:std   Treatment:None   Antibody:TR4   

Binding of Other Transcription Factors or Histone Marks at the TR4 Peaks

Select HeatmapLab:   Cellline:  

  • Comparison with the TR4 peaks in GM12878 cells generated by SYDH