TBP

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TATA box binding protein
TBP

PDB ID : 1TGH
PDB1TGH 1C9B 1JFI 1NVP 1CDW
HGNC(alias)TBP (TFIID,SCA17,GTF2D1)
Gene CardTBP
Entrez6908
RefSeqNM_001172085
UniProtP20226
UCSCBrowser view
WikipediaTBP
Protein FamilyTATA-box binding protein-like
Typegeneral Pol II associated factor, not site-specific
Ensembl Exp.n/a

Function

TBP (TATA-binding protein) is a subunit of the transcription factor TFIID. Binding of TFIID to the TATA box in the promoter is the first step in the formation of the pre-initiation complex (PIC) during RNA polymerase II-dependent transcription. TBP contains a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rates of transcription complex formation and transcription initiation. Typically 30-40 CAG repeats encode the polyglutamine tract, and an increase in the number of these repeats is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. TBP also is a component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. Two transcript variants encoding different isoforms have been found for this gene.

ENCODE ChIP-seq Datasets

Indicated in the matrix are the numbers of datasets specified by lab and cell line; when the number is greater than 1, multiple ChIP-seq experiments have been performed, some upon treatments. Click the numbers to download the data files on ChIP-seq peaks, alignments, etc.

+/-
Stanford
GM12878 1
H1-hESC 1
HeLa-S3 1
HepG2 1
K562 1


Average Profiles of Modified Histones around the Summit of ChIP-seq Peaks

Average histone modification profiles are shown for the [-2 kb, +2 kb] window around the summits of TF ChIP-seq peaks, separately for peaks that are proximal ([-1kb, +1kb]) to an annotated transcript (dashed lines) start sites and for peaks that are distal (more than 1kb) to all annotated transcripts (solid lines) start sites. Proximal profiles are arranged such that the transcriptional direction of the nearest transcript is toward the right. Histone modification data were generated by the Broad team, using antibodies to pull down modified histones followed by deep sequencing of the genomic DNA associated with the modified histones. Only histone modification data from the same cell line as the TF ChIP-seq data are shown.

Mouse over a curve to reveal its identity. Mouse over a histone modification in the legend to show its curves and gray out other histone modifications in the figures. Click a histone modification in the legend to toggle on/off its curve in all figures. Click the “Proximal” or “Distal” button in the legend to show only the average histone modification profiles anchored around ChIP-seq peaks that are proximal or distal to annotated transcripts.

Average Profiles of Nucleosomes around the Summit of ChIP-seq Peaks

Average nucleosome occupancy profiles are shown for the [-2 kb, +2 kb] window around the summits of TF ChIP-seq peaks, separately for peaks that are proximal to an annotated transcript (red lines) and for peaks that are distal to all annotated transcripts (blue lines), as defined in the previous section. Nucleosome positioning data in GM12878 and K562 were generated by the Stanford team, with micrococcal nuclease digestion of chromatin followed by deep sequencing of mononucleosomal DNA.

Motifs Enriched in the Top 500 ChIP-seq Peaks

The sequences of the top 500 TF ChIP-seq peaks were used to identify enriched motifs de novo, using the MEME-ChIP suite of tools. Five motifs are reported (M1 to M5), with motif name, sequence logo, and number of peaks out of the top 500 peaks that contain a site for the motif. The motifs are then used to scan the entire set of ChIP-seq peaks and the two flanking (control) regions using the FIMO tool, and two quantities are reported for bins of peaks sorted by their ChIP-seq q-values: percentage of the peaks that contain a site for the motif, and the distribution of the distances of the motif site to the summit of the peak.

Select MotifCell Line:   Lab:   Protocol:   Treatment:   Antibody:  

Cell Line:GM12878   Lab:Stanford   Protocol:IgG-mus   Treatment:None   Antibody:TBP   

Binding of Other Transcription Factors or Histone Marks at the TBP Peaks

Select HeatmapLab:   Cellline:  

  • Comparison with the TBP peaks in GM12878 cells generated by SYDH