STAT3

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signal transducer and activator of transcription 3 (acute-phase response factor)
STAT3
PDBsearch STAT3
HGNC(alias)STAT3 (APRF)
Gene CardSTAT3
Entrez6774
RefSeqNM_003150
UniProtP40763
UCSCBrowser view
WikipediaSTAT3
Protein Familyp53-like transcription factors
TypePol II TF
Ensembl Exp.Human

Function

STAT3 (signal transducer and activator of transcription 3) is a member of the STAT protein family. In response to cytokines and growth factors, receptor associated kinases phosphorylate STAT proteins, which then form homo- or heterodimers that translocate to the nucleus where they act as transcriptional activators and induce a cellular antiviral state. STAT3 mediates the expression of a variety of genes, and is important for cell growth, apopotosis, and possibly stem cell self-renewal. It can be activated by various ligands including interferons, HGF, EGF, PDGF, LIF, IL5, IL6, and leptin. The GTPase RAC1 binds and regulates STAT3 activity, and PIAS3 is a STAT3 inhibitor. It is required for the differentiation of TH17 helper T cells, which have been implicated in many autoimmune diseases. STAT3 loss-of-function mutations result in Hyperimmunoglobulin E syndrome, with symptoms including recurrent infections and disordered bone and tooth development. Constitutive activation of STAT3 is associated with various human cancers and commonly suggests poor prognosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described.

ENCODE ChIP-seq Datasets

Indicated in the matrix are the numbers of datasets specified by lab and cell line; when the number is greater than 1, multiple ChIP-seq experiments have been performed, some upon treatments. Click the numbers to download the data files on ChIP-seq peaks, alignments, etc.

+/-
Harvard
Stanford
GM12878 1
HeLa-S3 1
MCF10A-Er-Src 4


Average Profiles of Modified Histones around the Summit of ChIP-seq Peaks

Average histone modification profiles are shown for the [-2 kb, +2 kb] window around the summits of TF ChIP-seq peaks, separately for peaks that are proximal ([-1kb, +1kb]) to an annotated transcript (dashed lines) start sites and for peaks that are distal (more than 1kb) to all annotated transcripts (solid lines) start sites. Proximal profiles are arranged such that the transcriptional direction of the nearest transcript is toward the right. Histone modification data were generated by the Broad team, using antibodies to pull down modified histones followed by deep sequencing of the genomic DNA associated with the modified histones. Only histone modification data from the same cell line as the TF ChIP-seq data are shown.

Mouse over a curve to reveal its identity. Mouse over a histone modification in the legend to show its curves and gray out other histone modifications in the figures. Click a histone modification in the legend to toggle on/off its curve in all figures. Click the “Proximal” or “Distal” button in the legend to show only the average histone modification profiles anchored around ChIP-seq peaks that are proximal or distal to annotated transcripts.

Average Profiles of Nucleosomes around the Summit of ChIP-seq Peaks

Average nucleosome occupancy profiles are shown for the [-2 kb, +2 kb] window around the summits of TF ChIP-seq peaks, separately for peaks that are proximal to an annotated transcript (red lines) and for peaks that are distal to all annotated transcripts (blue lines), as defined in the previous section. Nucleosome positioning data in GM12878 and K562 were generated by the Stanford team, with micrococcal nuclease digestion of chromatin followed by deep sequencing of mononucleosomal DNA.

Motifs Enriched in the Top 500 ChIP-seq Peaks

The sequences of the top 500 TF ChIP-seq peaks were used to identify enriched motifs de novo, using the MEME-ChIP suite of tools. Five motifs are reported (M1 to M5), with motif name, sequence logo, and number of peaks out of the top 500 peaks that contain a site for the motif. The motifs are then used to scan the entire set of ChIP-seq peaks and the two flanking (control) regions using the FIMO tool, and two quantities are reported for bins of peaks sorted by their ChIP-seq q-values: percentage of the peaks that contain a site for the motif, and the distribution of the distances of the motif site to the summit of the peak.

Select MotifCell Line:   Lab:   Protocol:   Treatment:   Antibody:  

Cell Line:GM12878   Lab:Stanford   Protocol:IgG-mus   Treatment:None   Antibody:STAT3   

Binding of Other Transcription Factors or Histone Marks at the STAT3 Peaks

Select HeatmapLab:   Cellline:  

  • Comparison with the STAT3 peaks in GM12878 cells generated by SYDH